Staining:Article Title: Comparison of the modeling effects between two hemorrhoid models
Article Snippet: .. The study’s experimental pharmaceuticals and reagents included croton oil (GlpBio 1), olive oil (Sinopharm Chemical Reagent Co., Ltd., 20230807); pyridine (Xilong Scientific Co., Ltd., 240507D1); ether (Tianjin Kemiou Chemical Reagent Co., Ltd., 20230919); 2% isoflurane (RWD Life Science Co., Ltd., 20240902); 4% paraformaldehyde (biosharp BL539A); hematoxylin eosin high definition constant staining kit (Servicebio G1076); bovine serum albumin BSA (Servicebio GC305010 ); normal rabbit serum (concentrated type) (Servicebio G1209); histochemical kit DAB chromogenic reagent (Servicebio G1212); immunohistochemical tumor necrosis factor alpha (TNFα) primary antibody (Servicebio AF7014); immunohistochemical interleukin (IL)-2 primary antibody (Servicebio AF5105); immunohistochemical IL-6 primary antibody (Servicebio DF6087); RIPA lysate (Servicebio G2002-100ML); WB TNFα primary antibody (Servicebio GB12188-100); WB IL-2 primary antibody (Servicebio GB11114-100); WB IL-6 primary antibody (Servicebio GB11117-100); HRP goat anti-rabbit secondary antibody (Servicebio GB23303); HRP goat anti-mouse secondary antibody (Servicebio GB23301); prestained protein marker VII (Servicebio G2087-250UL); RNA extraction solution (Servicebio G3013); isopropanol (Sinopharm Chemical Reagent Co., Ltd., 80109218); absolute ethanol (Sinopharm Chemical Reagent Co., Ltd., 10009218); RNA lysis solution (Servicebio G3029); SweScript All-in-One RT SuperMix for qPCR (Servicebio G3337); 2 × universal blue SYBR Green qPCR Master Mix (Servicebio G3326). .. The laboratory equipment was as follows: pipette gun (Servicebio); microtome (Leica Microsystems (Shanghai) Co., Ltd., rm2016); embedding machine (Junjie Electronics Ltd., JB-P5); microscope (NIKON ECLIPSE E100, Nikon, Japan); cryomicrotome (CRYOSTAR NX50, Thermo Fisher Scientific Co., Ltd.); laser speckle contrast imaging system for blood flow (Pericam PSI System 2.0); single-arm stereotactic instrument for brain procedures (RWD Life Science Co., Ltd.); small animal anesthesia machine (RWD Life Science Co., Ltd.); 3D cryogenic grinder (Servicebio KZ-5F-3D); vortex mixer (Servicebio SMV-3500); handheld centrifuge (Servicebio SMC-5000F); PCR reaction plate (Servicebio PCR-9601-NS); PCR reaction tube (Servicebio PCR-02-FC); fluorescence quantitative PCR instrument (BIO rad CFX connect); PCR instrument (Eastwin Life Sciences, Inc. ETC811); electrophoresis apparatus (Servicebio SVT-2); high speed refrigerated centrifuge (Dragonlab D3024R); microplate reader (Rayto RT-6100); chemiluminescence instrument (Servicebio SCG-W3000); analysis software (Servicebio AIWBwellTM).
Immunohistochemical staining:Article Title: Comparison of the modeling effects between two hemorrhoid models
Article Snippet: .. The study’s experimental pharmaceuticals and reagents included croton oil (GlpBio 1), olive oil (Sinopharm Chemical Reagent Co., Ltd., 20230807); pyridine (Xilong Scientific Co., Ltd., 240507D1); ether (Tianjin Kemiou Chemical Reagent Co., Ltd., 20230919); 2% isoflurane (RWD Life Science Co., Ltd., 20240902); 4% paraformaldehyde (biosharp BL539A); hematoxylin eosin high definition constant staining kit (Servicebio G1076); bovine serum albumin BSA (Servicebio GC305010 ); normal rabbit serum (concentrated type) (Servicebio G1209); histochemical kit DAB chromogenic reagent (Servicebio G1212); immunohistochemical tumor necrosis factor alpha (TNFα) primary antibody (Servicebio AF7014); immunohistochemical interleukin (IL)-2 primary antibody (Servicebio AF5105); immunohistochemical IL-6 primary antibody (Servicebio DF6087); RIPA lysate (Servicebio G2002-100ML); WB TNFα primary antibody (Servicebio GB12188-100); WB IL-2 primary antibody (Servicebio GB11114-100); WB IL-6 primary antibody (Servicebio GB11117-100); HRP goat anti-rabbit secondary antibody (Servicebio GB23303); HRP goat anti-mouse secondary antibody (Servicebio GB23301); prestained protein marker VII (Servicebio G2087-250UL); RNA extraction solution (Servicebio G3013); isopropanol (Sinopharm Chemical Reagent Co., Ltd., 80109218); absolute ethanol (Sinopharm Chemical Reagent Co., Ltd., 10009218); RNA lysis solution (Servicebio G3029); SweScript All-in-One RT SuperMix for qPCR (Servicebio G3337); 2 × universal blue SYBR Green qPCR Master Mix (Servicebio G3326). .. The laboratory equipment was as follows: pipette gun (Servicebio); microtome (Leica Microsystems (Shanghai) Co., Ltd., rm2016); embedding machine (Junjie Electronics Ltd., JB-P5); microscope (NIKON ECLIPSE E100, Nikon, Japan); cryomicrotome (CRYOSTAR NX50, Thermo Fisher Scientific Co., Ltd.); laser speckle contrast imaging system for blood flow (Pericam PSI System 2.0); single-arm stereotactic instrument for brain procedures (RWD Life Science Co., Ltd.); small animal anesthesia machine (RWD Life Science Co., Ltd.); 3D cryogenic grinder (Servicebio KZ-5F-3D); vortex mixer (Servicebio SMV-3500); handheld centrifuge (Servicebio SMC-5000F); PCR reaction plate (Servicebio PCR-9601-NS); PCR reaction tube (Servicebio PCR-02-FC); fluorescence quantitative PCR instrument (BIO rad CFX connect); PCR instrument (Eastwin Life Sciences, Inc. ETC811); electrophoresis apparatus (Servicebio SVT-2); high speed refrigerated centrifuge (Dragonlab D3024R); microplate reader (Rayto RT-6100); chemiluminescence instrument (Servicebio SCG-W3000); analysis software (Servicebio AIWBwellTM).
Western Blot:Article Title: Comparison of the modeling effects between two hemorrhoid models
Article Snippet: .. The study’s experimental pharmaceuticals and reagents included croton oil (GlpBio 1), olive oil (Sinopharm Chemical Reagent Co., Ltd., 20230807); pyridine (Xilong Scientific Co., Ltd., 240507D1); ether (Tianjin Kemiou Chemical Reagent Co., Ltd., 20230919); 2% isoflurane (RWD Life Science Co., Ltd., 20240902); 4% paraformaldehyde (biosharp BL539A); hematoxylin eosin high definition constant staining kit (Servicebio G1076); bovine serum albumin BSA (Servicebio GC305010 ); normal rabbit serum (concentrated type) (Servicebio G1209); histochemical kit DAB chromogenic reagent (Servicebio G1212); immunohistochemical tumor necrosis factor alpha (TNFα) primary antibody (Servicebio AF7014); immunohistochemical interleukin (IL)-2 primary antibody (Servicebio AF5105); immunohistochemical IL-6 primary antibody (Servicebio DF6087); RIPA lysate (Servicebio G2002-100ML); WB TNFα primary antibody (Servicebio GB12188-100); WB IL-2 primary antibody (Servicebio GB11114-100); WB IL-6 primary antibody (Servicebio GB11117-100); HRP goat anti-rabbit secondary antibody (Servicebio GB23303); HRP goat anti-mouse secondary antibody (Servicebio GB23301); prestained protein marker VII (Servicebio G2087-250UL); RNA extraction solution (Servicebio G3013); isopropanol (Sinopharm Chemical Reagent Co., Ltd., 80109218); absolute ethanol (Sinopharm Chemical Reagent Co., Ltd., 10009218); RNA lysis solution (Servicebio G3029); SweScript All-in-One RT SuperMix for qPCR (Servicebio G3337); 2 × universal blue SYBR Green qPCR Master Mix (Servicebio G3326). .. The laboratory equipment was as follows: pipette gun (Servicebio); microtome (Leica Microsystems (Shanghai) Co., Ltd., rm2016); embedding machine (Junjie Electronics Ltd., JB-P5); microscope (NIKON ECLIPSE E100, Nikon, Japan); cryomicrotome (CRYOSTAR NX50, Thermo Fisher Scientific Co., Ltd.); laser speckle contrast imaging system for blood flow (Pericam PSI System 2.0); single-arm stereotactic instrument for brain procedures (RWD Life Science Co., Ltd.); small animal anesthesia machine (RWD Life Science Co., Ltd.); 3D cryogenic grinder (Servicebio KZ-5F-3D); vortex mixer (Servicebio SMV-3500); handheld centrifuge (Servicebio SMC-5000F); PCR reaction plate (Servicebio PCR-9601-NS); PCR reaction tube (Servicebio PCR-02-FC); fluorescence quantitative PCR instrument (BIO rad CFX connect); PCR instrument (Eastwin Life Sciences, Inc. ETC811); electrophoresis apparatus (Servicebio SVT-2); high speed refrigerated centrifuge (Dragonlab D3024R); microplate reader (Rayto RT-6100); chemiluminescence instrument (Servicebio SCG-W3000); analysis software (Servicebio AIWBwellTM).
Marker:Article Title: Comparison of the modeling effects between two hemorrhoid models
Article Snippet: .. The study’s experimental pharmaceuticals and reagents included croton oil (GlpBio 1), olive oil (Sinopharm Chemical Reagent Co., Ltd., 20230807); pyridine (Xilong Scientific Co., Ltd., 240507D1); ether (Tianjin Kemiou Chemical Reagent Co., Ltd., 20230919); 2% isoflurane (RWD Life Science Co., Ltd., 20240902); 4% paraformaldehyde (biosharp BL539A); hematoxylin eosin high definition constant staining kit (Servicebio G1076); bovine serum albumin BSA (Servicebio GC305010 ); normal rabbit serum (concentrated type) (Servicebio G1209); histochemical kit DAB chromogenic reagent (Servicebio G1212); immunohistochemical tumor necrosis factor alpha (TNFα) primary antibody (Servicebio AF7014); immunohistochemical interleukin (IL)-2 primary antibody (Servicebio AF5105); immunohistochemical IL-6 primary antibody (Servicebio DF6087); RIPA lysate (Servicebio G2002-100ML); WB TNFα primary antibody (Servicebio GB12188-100); WB IL-2 primary antibody (Servicebio GB11114-100); WB IL-6 primary antibody (Servicebio GB11117-100); HRP goat anti-rabbit secondary antibody (Servicebio GB23303); HRP goat anti-mouse secondary antibody (Servicebio GB23301); prestained protein marker VII (Servicebio G2087-250UL); RNA extraction solution (Servicebio G3013); isopropanol (Sinopharm Chemical Reagent Co., Ltd., 80109218); absolute ethanol (Sinopharm Chemical Reagent Co., Ltd., 10009218); RNA lysis solution (Servicebio G3029); SweScript All-in-One RT SuperMix for qPCR (Servicebio G3337); 2 × universal blue SYBR Green qPCR Master Mix (Servicebio G3326). .. The laboratory equipment was as follows: pipette gun (Servicebio); microtome (Leica Microsystems (Shanghai) Co., Ltd., rm2016); embedding machine (Junjie Electronics Ltd., JB-P5); microscope (NIKON ECLIPSE E100, Nikon, Japan); cryomicrotome (CRYOSTAR NX50, Thermo Fisher Scientific Co., Ltd.); laser speckle contrast imaging system for blood flow (Pericam PSI System 2.0); single-arm stereotactic instrument for brain procedures (RWD Life Science Co., Ltd.); small animal anesthesia machine (RWD Life Science Co., Ltd.); 3D cryogenic grinder (Servicebio KZ-5F-3D); vortex mixer (Servicebio SMV-3500); handheld centrifuge (Servicebio SMC-5000F); PCR reaction plate (Servicebio PCR-9601-NS); PCR reaction tube (Servicebio PCR-02-FC); fluorescence quantitative PCR instrument (BIO rad CFX connect); PCR instrument (Eastwin Life Sciences, Inc. ETC811); electrophoresis apparatus (Servicebio SVT-2); high speed refrigerated centrifuge (Dragonlab D3024R); microplate reader (Rayto RT-6100); chemiluminescence instrument (Servicebio SCG-W3000); analysis software (Servicebio AIWBwellTM).
RNA Extraction:Article Title: Comparison of the modeling effects between two hemorrhoid models
Article Snippet: .. The study’s experimental pharmaceuticals and reagents included croton oil (GlpBio 1), olive oil (Sinopharm Chemical Reagent Co., Ltd., 20230807); pyridine (Xilong Scientific Co., Ltd., 240507D1); ether (Tianjin Kemiou Chemical Reagent Co., Ltd., 20230919); 2% isoflurane (RWD Life Science Co., Ltd., 20240902); 4% paraformaldehyde (biosharp BL539A); hematoxylin eosin high definition constant staining kit (Servicebio G1076); bovine serum albumin BSA (Servicebio GC305010 ); normal rabbit serum (concentrated type) (Servicebio G1209); histochemical kit DAB chromogenic reagent (Servicebio G1212); immunohistochemical tumor necrosis factor alpha (TNFα) primary antibody (Servicebio AF7014); immunohistochemical interleukin (IL)-2 primary antibody (Servicebio AF5105); immunohistochemical IL-6 primary antibody (Servicebio DF6087); RIPA lysate (Servicebio G2002-100ML); WB TNFα primary antibody (Servicebio GB12188-100); WB IL-2 primary antibody (Servicebio GB11114-100); WB IL-6 primary antibody (Servicebio GB11117-100); HRP goat anti-rabbit secondary antibody (Servicebio GB23303); HRP goat anti-mouse secondary antibody (Servicebio GB23301); prestained protein marker VII (Servicebio G2087-250UL); RNA extraction solution (Servicebio G3013); isopropanol (Sinopharm Chemical Reagent Co., Ltd., 80109218); absolute ethanol (Sinopharm Chemical Reagent Co., Ltd., 10009218); RNA lysis solution (Servicebio G3029); SweScript All-in-One RT SuperMix for qPCR (Servicebio G3337); 2 × universal blue SYBR Green qPCR Master Mix (Servicebio G3326). .. The laboratory equipment was as follows: pipette gun (Servicebio); microtome (Leica Microsystems (Shanghai) Co., Ltd., rm2016); embedding machine (Junjie Electronics Ltd., JB-P5); microscope (NIKON ECLIPSE E100, Nikon, Japan); cryomicrotome (CRYOSTAR NX50, Thermo Fisher Scientific Co., Ltd.); laser speckle contrast imaging system for blood flow (Pericam PSI System 2.0); single-arm stereotactic instrument for brain procedures (RWD Life Science Co., Ltd.); small animal anesthesia machine (RWD Life Science Co., Ltd.); 3D cryogenic grinder (Servicebio KZ-5F-3D); vortex mixer (Servicebio SMV-3500); handheld centrifuge (Servicebio SMC-5000F); PCR reaction plate (Servicebio PCR-9601-NS); PCR reaction tube (Servicebio PCR-02-FC); fluorescence quantitative PCR instrument (BIO rad CFX connect); PCR instrument (Eastwin Life Sciences, Inc. ETC811); electrophoresis apparatus (Servicebio SVT-2); high speed refrigerated centrifuge (Dragonlab D3024R); microplate reader (Rayto RT-6100); chemiluminescence instrument (Servicebio SCG-W3000); analysis software (Servicebio AIWBwellTM).
Lysis:Article Title: Comparison of the modeling effects between two hemorrhoid models
Article Snippet: .. The study’s experimental pharmaceuticals and reagents included croton oil (GlpBio 1), olive oil (Sinopharm Chemical Reagent Co., Ltd., 20230807); pyridine (Xilong Scientific Co., Ltd., 240507D1); ether (Tianjin Kemiou Chemical Reagent Co., Ltd., 20230919); 2% isoflurane (RWD Life Science Co., Ltd., 20240902); 4% paraformaldehyde (biosharp BL539A); hematoxylin eosin high definition constant staining kit (Servicebio G1076); bovine serum albumin BSA (Servicebio GC305010 ); normal rabbit serum (concentrated type) (Servicebio G1209); histochemical kit DAB chromogenic reagent (Servicebio G1212); immunohistochemical tumor necrosis factor alpha (TNFα) primary antibody (Servicebio AF7014); immunohistochemical interleukin (IL)-2 primary antibody (Servicebio AF5105); immunohistochemical IL-6 primary antibody (Servicebio DF6087); RIPA lysate (Servicebio G2002-100ML); WB TNFα primary antibody (Servicebio GB12188-100); WB IL-2 primary antibody (Servicebio GB11114-100); WB IL-6 primary antibody (Servicebio GB11117-100); HRP goat anti-rabbit secondary antibody (Servicebio GB23303); HRP goat anti-mouse secondary antibody (Servicebio GB23301); prestained protein marker VII (Servicebio G2087-250UL); RNA extraction solution (Servicebio G3013); isopropanol (Sinopharm Chemical Reagent Co., Ltd., 80109218); absolute ethanol (Sinopharm Chemical Reagent Co., Ltd., 10009218); RNA lysis solution (Servicebio G3029); SweScript All-in-One RT SuperMix for qPCR (Servicebio G3337); 2 × universal blue SYBR Green qPCR Master Mix (Servicebio G3326). .. The laboratory equipment was as follows: pipette gun (Servicebio); microtome (Leica Microsystems (Shanghai) Co., Ltd., rm2016); embedding machine (Junjie Electronics Ltd., JB-P5); microscope (NIKON ECLIPSE E100, Nikon, Japan); cryomicrotome (CRYOSTAR NX50, Thermo Fisher Scientific Co., Ltd.); laser speckle contrast imaging system for blood flow (Pericam PSI System 2.0); single-arm stereotactic instrument for brain procedures (RWD Life Science Co., Ltd.); small animal anesthesia machine (RWD Life Science Co., Ltd.); 3D cryogenic grinder (Servicebio KZ-5F-3D); vortex mixer (Servicebio SMV-3500); handheld centrifuge (Servicebio SMC-5000F); PCR reaction plate (Servicebio PCR-9601-NS); PCR reaction tube (Servicebio PCR-02-FC); fluorescence quantitative PCR instrument (BIO rad CFX connect); PCR instrument (Eastwin Life Sciences, Inc. ETC811); electrophoresis apparatus (Servicebio SVT-2); high speed refrigerated centrifuge (Dragonlab D3024R); microplate reader (Rayto RT-6100); chemiluminescence instrument (Servicebio SCG-W3000); analysis software (Servicebio AIWBwellTM).
Real-time Polymerase Chain Reaction:Article Title: Comparison of the modeling effects between two hemorrhoid models
Article Snippet: .. The study’s experimental pharmaceuticals and reagents included croton oil (GlpBio 1), olive oil (Sinopharm Chemical Reagent Co., Ltd., 20230807); pyridine (Xilong Scientific Co., Ltd., 240507D1); ether (Tianjin Kemiou Chemical Reagent Co., Ltd., 20230919); 2% isoflurane (RWD Life Science Co., Ltd., 20240902); 4% paraformaldehyde (biosharp BL539A); hematoxylin eosin high definition constant staining kit (Servicebio G1076); bovine serum albumin BSA (Servicebio GC305010 ); normal rabbit serum (concentrated type) (Servicebio G1209); histochemical kit DAB chromogenic reagent (Servicebio G1212); immunohistochemical tumor necrosis factor alpha (TNFα) primary antibody (Servicebio AF7014); immunohistochemical interleukin (IL)-2 primary antibody (Servicebio AF5105); immunohistochemical IL-6 primary antibody (Servicebio DF6087); RIPA lysate (Servicebio G2002-100ML); WB TNFα primary antibody (Servicebio GB12188-100); WB IL-2 primary antibody (Servicebio GB11114-100); WB IL-6 primary antibody (Servicebio GB11117-100); HRP goat anti-rabbit secondary antibody (Servicebio GB23303); HRP goat anti-mouse secondary antibody (Servicebio GB23301); prestained protein marker VII (Servicebio G2087-250UL); RNA extraction solution (Servicebio G3013); isopropanol (Sinopharm Chemical Reagent Co., Ltd., 80109218); absolute ethanol (Sinopharm Chemical Reagent Co., Ltd., 10009218); RNA lysis solution (Servicebio G3029); SweScript All-in-One RT SuperMix for qPCR (Servicebio G3337); 2 × universal blue SYBR Green qPCR Master Mix (Servicebio G3326). .. The laboratory equipment was as follows: pipette gun (Servicebio); microtome (Leica Microsystems (Shanghai) Co., Ltd., rm2016); embedding machine (Junjie Electronics Ltd., JB-P5); microscope (NIKON ECLIPSE E100, Nikon, Japan); cryomicrotome (CRYOSTAR NX50, Thermo Fisher Scientific Co., Ltd.); laser speckle contrast imaging system for blood flow (Pericam PSI System 2.0); single-arm stereotactic instrument for brain procedures (RWD Life Science Co., Ltd.); small animal anesthesia machine (RWD Life Science Co., Ltd.); 3D cryogenic grinder (Servicebio KZ-5F-3D); vortex mixer (Servicebio SMV-3500); handheld centrifuge (Servicebio SMC-5000F); PCR reaction plate (Servicebio PCR-9601-NS); PCR reaction tube (Servicebio PCR-02-FC); fluorescence quantitative PCR instrument (BIO rad CFX connect); PCR instrument (Eastwin Life Sciences, Inc. ETC811); electrophoresis apparatus (Servicebio SVT-2); high speed refrigerated centrifuge (Dragonlab D3024R); microplate reader (Rayto RT-6100); chemiluminescence instrument (Servicebio SCG-W3000); analysis software (Servicebio AIWBwellTM).
SYBR Green Assay:Article Title: Comparison of the modeling effects between two hemorrhoid models
Article Snippet: .. The study’s experimental pharmaceuticals and reagents included croton oil (GlpBio 1), olive oil (Sinopharm Chemical Reagent Co., Ltd., 20230807); pyridine (Xilong Scientific Co., Ltd., 240507D1); ether (Tianjin Kemiou Chemical Reagent Co., Ltd., 20230919); 2% isoflurane (RWD Life Science Co., Ltd., 20240902); 4% paraformaldehyde (biosharp BL539A); hematoxylin eosin high definition constant staining kit (Servicebio G1076); bovine serum albumin BSA (Servicebio GC305010 ); normal rabbit serum (concentrated type) (Servicebio G1209); histochemical kit DAB chromogenic reagent (Servicebio G1212); immunohistochemical tumor necrosis factor alpha (TNFα) primary antibody (Servicebio AF7014); immunohistochemical interleukin (IL)-2 primary antibody (Servicebio AF5105); immunohistochemical IL-6 primary antibody (Servicebio DF6087); RIPA lysate (Servicebio G2002-100ML); WB TNFα primary antibody (Servicebio GB12188-100); WB IL-2 primary antibody (Servicebio GB11114-100); WB IL-6 primary antibody (Servicebio GB11117-100); HRP goat anti-rabbit secondary antibody (Servicebio GB23303); HRP goat anti-mouse secondary antibody (Servicebio GB23301); prestained protein marker VII (Servicebio G2087-250UL); RNA extraction solution (Servicebio G3013); isopropanol (Sinopharm Chemical Reagent Co., Ltd., 80109218); absolute ethanol (Sinopharm Chemical Reagent Co., Ltd., 10009218); RNA lysis solution (Servicebio G3029); SweScript All-in-One RT SuperMix for qPCR (Servicebio G3337); 2 × universal blue SYBR Green qPCR Master Mix (Servicebio G3326). .. The laboratory equipment was as follows: pipette gun (Servicebio); microtome (Leica Microsystems (Shanghai) Co., Ltd., rm2016); embedding machine (Junjie Electronics Ltd., JB-P5); microscope (NIKON ECLIPSE E100, Nikon, Japan); cryomicrotome (CRYOSTAR NX50, Thermo Fisher Scientific Co., Ltd.); laser speckle contrast imaging system for blood flow (Pericam PSI System 2.0); single-arm stereotactic instrument for brain procedures (RWD Life Science Co., Ltd.); small animal anesthesia machine (RWD Life Science Co., Ltd.); 3D cryogenic grinder (Servicebio KZ-5F-3D); vortex mixer (Servicebio SMV-3500); handheld centrifuge (Servicebio SMC-5000F); PCR reaction plate (Servicebio PCR-9601-NS); PCR reaction tube (Servicebio PCR-02-FC); fluorescence quantitative PCR instrument (BIO rad CFX connect); PCR instrument (Eastwin Life Sciences, Inc. ETC811); electrophoresis apparatus (Servicebio SVT-2); high speed refrigerated centrifuge (Dragonlab D3024R); microplate reader (Rayto RT-6100); chemiluminescence instrument (Servicebio SCG-W3000); analysis software (Servicebio AIWBwellTM).
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